Studies on Bolynucleotides
نویسنده
چکیده
Purified phenylalanine transfer RNA (tRNA) from yeast has been degraded with pancreatic ribonuclease, and the fragments obtained have been separated and identified. The 3’-terminal nucleoside is cytidine, and the 5’4errninal dinucleotide is pGpCp. Fourteen minor nucleosides are present, including 7-methylguanosine, 2’-0-methylcytidine, and a nucleoside the identity of which is not established. The sequences involving some of the minor nucleosides, N2dimethyl-GpCp, GpTp, and ApGp-dihydro-Up, are common to many other tRNAs. The largest oligonucleotide produced is an octanucleotide with the sequence GpGpGpApGpApGPCP.
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